Fig. 3.

Comparisons of phenotype and peripheral residency between VM and naïve CD8 T cells during primary L. monocytogenes infection. (A and B) Short-lived effector (KLRG1⁺ CD127^lo) and memory precursor (KLRG1⁻ CD127^hi) phenotype of responding VM and naïve CD8 T cells, during effector and memory phase following LM-OVA infection. Frequencies of phenotypic subsets were determined on Ova/K^b-tetramer⁺ donor Vβ5 CD8 T cells at the indicated times postinfection. Line graphs show mean ± SD. (C) Central memory (CD62L⁺) differentiation of responding VM and naïve donor Vβ5 CD8 T cells. The frequency of CD62L⁺ cells in cotransfered naïve and VM populations is shown. (D) Ratio between Ova/K^b-tetramer-specific VM and naïve Vβ5 CD8 T cells in indicated tissues and blood at 50–60 d post-LM-OVA infection. (Blood contamination in each tissue was excluded as described in SI Materials and Methods). For all experiments, data were compiled from three independent experiments, except day 22 p.i. (two independent experiments; six mice total). Statistical significance between groups is indicated (***P < 0.001; **P < 0.01; *P < 0.05, whereas NS, not significant, is used to denote P values >0.05, Student t test).