Fig. 4.

The light response of ROC15-LUC reporters in the roc114-1 background. A ROC15-LUC reporter strain having the roc114-1 background was obtained by genetic crosses (SI Appendix, Fig. S10). WT siblings were used as a control. Cell cultures were prepared in black 24-well plates as described in Fig. 2. Cells were exposed to white (20 μmol⋅m⁻²⋅s⁻¹, 3 min), blue (20 μmol⋅m⁻²⋅s⁻¹, 3 min), or red (20 μmol⋅m⁻²⋅s⁻¹, 0.5 min) light pulses (A) or to red light pulses (200 μmol⋅m⁻²⋅s⁻¹) of different durations (B). Arrows indicate the times when the light pulses were given. The values just before the light pulse were normalized to 100. Each point represents the mean ± SD of the normalized bioluminescence traces from three independent cultures. Similar results were obtained in two independent experiments (A and B).