Fig. 1.
In situ analysis of BslA localization in the complex colony biofilm. Confocal scanning laser microscopy images of cross-sections through complex colonies formed by either (A and C) wild-type cells (3610, sacA::Phy-spank-gfp; NRS1473) or (B and D) the bslA mutant strain (3610, bslA::cat, sacA::Phy-spank-gfp; NRS3812). The smaller images show the region highlighted by the white box at higher magnification. Fluorescence from the GFP within the cells is shown in green in the large panels and in the merged images and the fluorescence associated with DyLight594, representing immuno-labeled BslA staining, is shown in red. (Scale bar, 50 µm.)