Fig. 3.

Immunization with MAPS complex elicits antibody, Th1, and Th17 responses to protein antigens. (A) Mice were immunized with adjuvant alone, a mixture of CPS3 and TB antigens (mixture), or MAPS complex prepared with biotinylated serotype-3 pneumococcal CPS (BCPS3) and four fusion TB antigens, rhavi-ESAT6/Cfp10, rhavi-TB9.8/TB10.4, rhavi-MPT64, and rhavi-MPT83 [1:1:1:1, (wt/wt)]. (B–D) The MAPS complexes elicited significantly higher antibody titers against target proteins (B) and robust Th1 and Th17 responses from peripheral blood, as measured by production of IFN-γ (C) and IL-17A (D). Bars represent mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.0001. (E) Effect of depletion of CD4⁺ and CD8⁺ T cells on IFN-γ (Left) and IL-17A production (Right). Open bars represent no Ag; gray bars, mixture; black bars, MAPS.