Fig. 2.

Lignin levels in the Medicago cad1-1 and cad1-2 mutants and a complemented cad1-1 line. (A) Acetyl bromide lignin content of stems (fifth to eighth internodes) of 4-mo-old M. truncatula wild type (WT), cad1-1 and cad1-2 mutants, and cad1-1 line complemented with the wild-type Medicago CAD1 sequence (Comp). (B) Thioacidolysis yields of p-hydroxyphenyl (H), guaiacyl (G), and syringyl (S) lignin monomers from the above lines. (C) Quantification of wall-bound coniferaldehyde-derived indene compounds. Bars show means and SDs; n = 6. nd, not detected. CWR, cell wall residue.