Table 1.

Cryo-EM data collection and image reconstruction

Sample	Defocus,* μm	Symmetry of the model and reconstruction	No. of particles	Resolution,† Å
SINV (pH 6.4)	1.10–2.89	Icosahedral	5,670	14.5
SINV–liposome (pH 6.4)	1.08–4.01	2-fold	442	∼30.0
		3-fold	237	∼30.0
		5-fold	196	∼30.0
		q3-fold‡	550	∼30.0

^*Defocus levels were determined using RobEM (http://cryoem.ucsd.edu/programs.shtm).

^†The resolution of the SINV (pH 6.4) map was assessed using Fourier shell correlation with a threshold value of 0.5. The reconstruction maps of the SINV–liposome complexes were computed to 25.0-Å resolution. The resolutions of these maps were estimated to be ∼30 Å because the virus membrane bilayers (32-Å peak-to-peak distance) were clearly distinguishable.

^‡Reconstruction was computed without imposing any symmetry.