Figure 2.

Translocation of HMGB1 induced by pressure overload and hypertrophic stimulation. (A) Decrease in nuclear localization of HMGB1 after pressure overload. Data are expressed as means ± SEM. ^†P < 0.05 vs WT TAC mice. (B) HMGB1 expression in the nucleus and cytoplasm of neonatal rat cardiomyocytes after ET-1 (100 nM) stimulation. Data are expressed as means means ± SEM. ^†P < 0.05 vs control. (C) Lysates of neonatal rat cardiomyocytes treated with or without ET-1 were immunoprecipitated with an anti-acetyl-lysine antibody and immunoblotted against an anti-HMGB1 antibody. (D) Lysates of neonatal rat cardiomyocytes following stimulus with ET-1 (100 nM) were immunoprecipitated with an anti-acetyl-lysine antibody and immunoblotted against HMGB1.