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. 2013 May 25;99(4):657–664. doi: 10.1093/cvr/cvt128

Figure 3.

Figure 3

Protective effect of nuclear HMGB1 on ET-1 induced hypertrophy. (A) Increase in ANP and BNP promoter activity by HMGB1 SiRNA transfection after ET-1 stimulation. Data represent means ± SEM from three independent experiments; †P < 0.05. (B) Suppressed ANP and BNP promoter activity after ET-1 stimulation in HMGB1 vector transfected cardiomyocytes. Data represent means ± SEM; †P < 0.05. (C) Effect of HMGB1 overexpression on the cell surface area of neonatal rat cardiomyocytes after ET-1 stimulation. Cardiomyocytes were transfected with pmax-GFP with either an HMGB1 expression vector or a control vector, and incubated for 48 h with or without ET-1. Scale bar = 20 μm.