Figure 4.

Preserved amounts of nuclear HMGB1 prevented DNA damage induced by pressure overload. (A) Acetylation and translocation of cardiac HMGB1 from the nucleus to the cytoplasm after pressure overload. (B) Immunohistochemical staining of heart tissues with an anti-8-OHdG antibody 4 weeks after TAC and quantification of histological data (n = 5). Scale bar, 50 mm. (C) Results were normalized by arbitrarily setting the area of the 8-OHDG positive cells in HMGB1-TG TAC mice to 1.0. Bars represent means ± SEM. (n = 5). ^†P < 0.05 vs. WT TAC mice.