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Scheme of Langendorff experiments performed. For infarct analysis (n = 6 per group), hearts were randomly assigned to receive (A) sub-lethal (20 min regional ischaemia), (B) lethal (35 min regional ischaemia), or (C) IPC (three cycles of 5 min ischaemia/10 min reperfusion prior to lethal ischaemia), all followed by 60 min reperfusion. Hearts were collected for WB analysis either at baseline or following IPC.
