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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Psychopharmacol. 2012 Jul 11;26(10):1333–1347. doi: 10.1177/0269881112450786

Figure 4. Ca2+ -independent and -dependent isoforms of PKC regulate 5-HT2A receptor mRNA levels in CLU213 cells.

Figure 4

(A) Inhibition of Ca2+ -independent and –dependent isoforms of PKC (GF 109203X) enhanced basal levels of 5-HT2A receptor mRNA. *p<0.05, significant effect of GP 1a treatment, GF 109203X pretreatment, and GP 1a/GF 109203X treatment compared to vehicle-treated controls. (B) Inhibition of Ca2+ -dependent isoforms of PKC (Go 6967) enhanced basal levels of 5-HT2A receptor mRNA. **p<0.01, significant effect of GP 1a treatment, Go 6967 pretreatment, and GP 1a/Go 6967 treatment compared to vehicle-treated controls. (C) Activation of Ca2+ -independent and -dependent isoforms of PKC (PDBu) prevented GP 1a-induced increases in 5-HT2A receptor mRNA. *p<0.05, significant effect of GP 1a treatment compared to vehicle-treated controls. #p<0.05, significant effect of PDBu pretreatment on GP 1a-induced increases in 5-HT2A receptor mRNA. (D) Activation of Ca2+ -dependent isoforms of PKC did not prevent GP 1a-induced increases in 5-HT2A receptor mRNA. **p<0.01, significant effect of GP 1a treatment compared to vehicle-treated controls. The data represent mean ± SEM (n=3).