Fig. 3.

Morphologies of droplet–hydrogel composites with and without exposure to ultrasound (US). Representative images of transmitted light (488 nm, shown in grayscale) and maximum projections from 100 μm stacks of confocal fluorescence images (AF647-fibrinogen, shown in red) depict ADV-induced changes in scaffold architecture for 5 mg ml⁻¹ and 10 mg ml⁻¹ fibrin hydrogels doped with 0%, 1% or 5% (v/v) double emulsion. Panels at left show the morphology of fibrin hydrogels without droplets that were exposed to ultrasound; sham controls appeared similar. Due to the curved surfaces of the samples, we observed gradients in the intensity of transmitted light for the hydrogels without emulsion. Scale bars = 200 μm.