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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Acta Biomater. 2013 Mar 25;9(7):7399–7409. doi: 10.1016/j.actbio.2013.03.027

Fig. 7.

Fig. 7

Effects of ultrasound (US) exposure on HUVECs in droplet–hydrogel composites. The metabolic activity (A) and release of AF647 fibrinogen (B) from HUVEC composites were measured 2 days after ADV. Data are shown as mean ± standard deviation for n = 5 (metabolic activity) and n = 4 (release). #p < 0.05 vs. sham control. CMFDA (blue)/PI (green) labeling of HUVECs in 5 mg ml−1 fibrin constructs demonstrates proximity of viable cells to bubbles formed by ADV (C). The percentage of viable cells (mean ± standard deviation; n = 3) for each condition is given in the upper right. The right column displays zoomed-in panels from the center column (denoted by the boxed regions). Viable (white arrows) and necrotic cells (yellow arrows) are observed adjacent to the bubbles. Scale bars = 200 μm.