Table 3. Binding of ATROSAB and TNF to human TNFR1 mutants in ELISA.

TNFR1	ATROSAB binding	TNF binding
Mutant	EC50 (nM)	EC50 (nM)
Wt	0.27	0.19
P23S	11.0	0.16
Q24K	0.23	0.17
H66Y	0.18	0.14
R68A	5.0	0.16
H69Q	8.6	0.15
H69A	118.5	0.14
R92K	0.12	0.08

Details of binding studies are described in Materials and Methods.