Table 1. List of primers used in HIV-1 genotypic drug-resistance testing.

Target region	Amplicon		Primers
	Size (bp)	Positiona	Reaction	Direction	Name	Nucleotide sequence (5′ to 3′)
Protease	424	2,168 to 2,591	RT-PCR	Forward	DRPRO5	AGA CAG GYT AAT TTT TTA GGG A
				Reverse	DRPRO2L	TAT GGA TTT TCA GGC CCA ATT TTT GA
			Nested PCR	Forward	DRPRO1M	AGA GCC AAC AGC CCC ACC AG
				Reverse	DRPRO6	ACT TTT GGG CCA TCC ATT CC
Reverse transcriptase	838	2,510 to 3,347	RT-PCR	Forward	DRRT1L	ATG ATA GGG GGA ATT GGA GGT TT
				Reverse	DRRT4L	TAC TTC TGT TAG TGC TTT GGT TCC
			Nested PCR	Forward	DRRT7L	GAC CTA CAC CTG TCA ACA TAA TTG G
				Reverse	DRRT6L	TAA TCC CTG CAT AAA TCT GAC TTG C

bp, base pairs; PCR, polymerase chain reaction; and RT-PCR, reverse transcription and polymerase chain reaction.

^aAmplicon positions in the reference HIV-1 HXB2 sequence are represented.