Figure 1. Analytical SEC of 14-3-3ζ, unphosphorylated HspB6 (B6) or phosphorylated HspB6 (pB6) and their mixtures in the presence or in the absence of phosphate (Pi).
(A) Elution profiles of the mixture of 14-3-3ζ and either of unphosphorylated (B6, curve 1) or phosphorylated (pB6, curve 2) HspB6 (28 µM of each) performed in Tris-buffer or of the mixture of 14-3-3ζ and pHspB6 performed in phosphate-containing buffer (curve 3). Representative results of four independent experiments are presented. Protein composition of the fractions was analyzed by SDS gel-electrophoresis (B). Positions of 14-3-3 (~30 kDa) and HspB6 (~20 kDa) are indicated by arrows.