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. 2013 Sep;94(3):399–407. doi: 10.1189/jlb.0512249

Figure 5. Blockade of Panx1 hemichannels abolishes HIV replication in CD4+ T lymphocytes.

Figure 5.

(A and B) ELISA for HIV-p24 release in uninfected (squares) or HIVADA-infected (circles) CD4+ T lymphocytes. (A) Blockade of Panx1 hemichannels by using mimetic peptides (10Panx1, 200 μM, upright triangles) or (B) knockdown of Panx1 by using siRNA (upright triangles, 10 nM) abolished HIV replication to almost control, undetectable levels (squares). Scrambled peptides and siRNAscr (downward triangles) did not alter viral replication. (C) Summary of three independent experiments after 7 days postinfection of CD4+ T lymphocytes. HIV-p24 release into the media in the presence or absence of the Cx43 hemichannel blocker Cx43E2 (1:500 dilution), Panx1 hemichannel blocker, 10Panx1 (200 μM), and scrPanx1 (200 μM) are shown (*P<0.005). No differences in viability were observed among the different conditions. Each value corresponds to the mean ± sd of the Etd uptake intensity of at least 20 cells/time-point (n=4 for all experiments).