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. 2013 Aug 19;8(8):e72015. doi: 10.1371/journal.pone.0072015

Figure 9. UBIAD1 might be targeted to the Golgi by COPII-mediated mechanism.

Figure 9

A, HEK293 cells were transfected with pUBIAD1-EGFP and co-transfected with Ds Red-ER (ER marker) and Ds Red-Golgi (Golgi marker), respectively. Following 24 hours of transfection, transfected HEK293 cells were treated with DMEM (1normal, as control) and brefeldin A (BFA, 5 ug/mL) for 0.5 hr (2), 1 hour (3) and 5 hours (4). Line arrow: ER; Arrow head: the Golgi. B, Substitution of Arginine 54 (R54) to lysine (K) does not affect the Golgi localization of UBIAD1. UBIAD1-EGFP was site-directed mutagenised by substituting the Arginine 54 (R54) to lysine. The mutant clone was transfected into the HEK293 and T24 cells. C, A model representing the putative interactions between RPWS of the UBIAD1 and the GTP of the Sar1 subunit of the COPII complex; Dotted lines represent the possible interactions. Experiments were repeated at least three times. Size bar represents 10 µm.