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. 2013 Aug 19;8(8):e71232. doi: 10.1371/journal.pone.0071232

Table 1. Primers used in this study.

Primer name Sequences (5′–3′) Application Source
Rbc5a CTGTTCGACCGCAACMTCACCGATGG Dinoflagellate rbcII PCR (forward) [5]
Rbc3a CCAAGCTTCTCCTTCCAKCCHGGRTADAT Dinoflagellate rbcII PCR (reverse) [5]
Dino-SL TCCGTAGCCATTTTGGCTCAAG Dinoflagellate mRNA 5′ end cDNA synthesis and PCR (forward) [16]
Pdrbc5b GCAACTTCCCGAAGCAGTTCCTGCA Pdrbc 3′ end isolation This study
Pdrbc3b GTACTTGCCACGCGCGATCATCTCA Pdrbc 5′ end isolation This study
GeneRacer oligo-dT GCTGTCAACGATACGCTACGTAACGGCATGACAGTG(T)24 cDNA library construct Invitrogen
GeneRacer3 GCTGTCAACGATACGCTACGTAACG cDNA library construct Invitrogen
Regular oligo-dT (T)16V (V = A,G,C) cDNA library construct [5]
Modified random N9 GAGACTATGCGCCTTGCCAGCCCGCTCAGTAATACGACTCACTATAGGGAGNNNNNNNNN cDNA library construct [15]
Rbc5F CTGGACYAGTCGAGCCGYTAYGCKGA Pdrbc PCR (forward) This study
Rbc3R CTCCTTSTTCTGCCARTTGAAGCTRGC Pdrbc PCR (reverse) This study
Rbc5H GGCTGGAAGGAGAAGCTCGGCTACAC Pdrbc PCR (forward) This study
Rbc3H CGGTCGGGTAYGCAATyTTCATCTC Pdrbc PCR (reverse) This study
Rbc-QF GCGAAGACCCATGAGGAGATCAAG Pdrbc qPCR (forward) This study
Rbc-QR TCAACTCAACTCCTTCTTCTGCCAAT Pdrbc qPCR (reverse) This study
Pdong-cal-QF AGTTCAAGGAGGCGTTCTCTTTGTTC P. donghaiense calmodulin qPCR (forward) This study
Pdong-cal-QR CCATCAAGGACAAGAACTCGGGAAAG P. donghaiense calmodulin qPCR (reverse) This study
Pdong-gapdh-QF GTGTTCCTYACCGACGAGAAGATC P. donghaiense gapdh qPCR (forward) This study
Pdong-gapdh-QR3 CGCARTTCATGTCAGTCTTGTAGG P. donghaiense gapdh qPCR (reverse) This study