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. Author manuscript; available in PMC: 2014 Jul 17.
Published in final edited form as: J Am Chem Soc. 2013 Jul 9;135(28):10426–10432. doi: 10.1021/ja402832z

Table 2.

Kinetic parameters for PTE variants with paraoxon and demeton-Sa.

Paroxon Demeton-S
Variant k cat
(s−1)		 Km
(µM)		 kcat/Km
(M−1s−1)		 kcat
(s−1)		 Km
(mM)		 kcat/Km
(M−1s−1)
WT 6700 100 6.7 × 107 1.4 1.2 1.1 × 103
QF 41 5.3 7.7 × 106 6.3 2.6 2.7 × 103
LQF 72 11.1 6.5 × 106 4.4 3.3 1.3 × 103
VQF 108 10.5 1.0 × 107 10 6.1 1.7 × 103
LQFL 90 11 8.2 × 106 5.2 3.1 1.7 × 103
VQFL 66 5.4 1.2 × 107 4.2 3.9 1.1 × 103
CVQFL 38 5.6 6.8 × 106 6.1 2.5 2.4 × 103
VRN-VQFL 116 8 1.5 × 107 7.1 2.8 2.5 × 103
VRNGS-VQFL 227 8.5 2.7 × 107 6.8 3.1 2.2 × 103
L7ep-1 1590 116 1.4 × 107 0.12 1.0 1.2 × 102
L7ep-2 245 93 2.6 × 106 73 0.84 8.7 × 104
L7ep-3 146 11.2 1.3 × 107 3.4 2.4 1.4 × 103
L7ep-2a 243 46 5.3 × 106 32 0.58 5.4 × 104
L7ep-2b 280 13.5 2.1 × 107 53 0.57 9.2 × 104
L7ep-3a 85 7.2 1.2 × 107 1.8 2.5 7.4 × 102
a

Standard errors from fits of the data fit to equation 1 are less than 10% of the stated values.