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. 2013 Aug 19;8(8):e71543. doi: 10.1371/journal.pone.0071543

Figure 5. Effects of NO on transcriptional expression of several K+/Na+ balance related genes.

Figure 5

Expressions of AKT1 (A), SOS1 (C), NHX1 (E), HA1 (G) and VHA-c1 (I) genes in K. obovata roots in response to 400 mM NaCl and various concentrations of SNP for 15 days. Shown in B, D, F, H and J, respectively, stand for the gene expressions of AKT1, SOS1, NHX1, HA1, VHA-c1 in the roots of K. obovata seedlings grown under the following Hoagland solution-based solutions for 15 days: Control (only Hoagland solution), NaCl (400 mM), SNP (100 µM), NaCl + SNP (400 mM NaCl+100 µM SNP), cPTIO+L-NNA (200 µM cPTIO+100 µM L-NNA), NaCl+cPTIO+L-NNA (400 mM NaCl+200 µM cPTIO+100 µM L-NNA). The relative transcriptional expression of each gene was performed by normalization with 18S rRNA, a reference gene of K. obovata. Mean values and SE were calculated from three independent experiments. For each parameter, bars with different letters indicate significant difference at P<0.05.