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. 2013 Aug 19;202(4):667–683. doi: 10.1083/jcb.201301072

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© 2013 Matkovic et al.

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Figure 6. — Electrophysiological characterization of brp isoform mutant NMJs. (A) Example traces and quantification of evoked excitatory junctional currents (eEJC) recorded in 1.5 mM Ca²⁺ at 0.2 Hz. n is as indicated in the bars. (B) Rise time and the decay time constants (τ) of eEJCs were unchanged (rise time: control, 1.23 ± 0.06, n = 21; brp^Δ170, 1.40 ± 0.07, P > 0.05, n = 19; brp^Δ190, 1.37 ± 0.06, P > 0.05, n = 19; τ: control, 6.74 ± 0.17; brp^Δ170, 6.80 ± 0.19, P > 0.05; brp^Δ190, 7.21 ± 0.30, P > 0.05; one-way ANOVA Tukey’s posttest). (C, left) Example traces of mEJCs. (right) mEJC amplitude was unchanged in both brp isoform mutants (control: −0.93 ± 0.04, n = 19; brp^Δ170: −0.86 ± 0.04, P > 0.05, n = 16; brp^Δ190: −0.95 ± 0.03, P > 0.05, n = 16; one-way ANOVA Tukey’s posttest). (D) mEJC frequency was increased in brp^Δ170mutants (control: 0.74 ± 0.08, n = 19; brp^Δ170: 1.47 ± 0.20, P < 0.01, n = 16; brp^Δ190: 1.03 ± 0.13, P > 0.05, n = 16; one-way ANOVA Tukey’s posttest). (E) mEJC kinetics were not changed in both brp isoform mutants (mEJC rise time: control, 1.17 ± 0.06, n = 19; brp^Δ170, 1.27 ± 0.11, P > 0.05, n = 15; brp^Δ190, 1.15 ± 0.04, n = 16, P > 0.05; mEJC τ: control, 6.73 ± 0.20, P > 0.05; brp^Δ170, 6.55 ± 0.18, P > 0.05; brp^Δ190, 6.86 ± 0.27, P > 0.05; one-way ANOVA Tukey’s posttest). (F) Normalized amplitudes at a 10-Hz stimulation for 10 s. Control and both mutants showed no difference in depression kinetics (control, n = 14; brp^Δ170, n = 6; brp^Δ190, n = 8). (G) Paired-pulse ratio at two different ISIs of 10 and 30 ms was not different between control and mutants (ISI 10 ms: control, 1.36 ± 0.06, n = 19; brp^Δ170, 1.44 ± 0.07, P > 0.05, n = 15; brp^Δ190, 1.40 ± 0.05, P > 0.05, n = 16; ISI 30 ms: control, 1.19 ± 0.04, n = 15; brp^Δ170, 1.35 ± 0.07, P > 0.05, n = 13; brp^Δ190, 1.29 ± 0.06, P > 0.05, n = 18; one-way ANOVA Tukey’s posttest). Example traces for 30-ms ISI rescaled to the amplitude of the first pulse (dotted line) are shown. All panels show mean values and errors bars representing SEMs (unless otherwise noted). *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; n.s., P > 0.05.