Table 2.
Septation of vacuoles during hyperosmotic adaptation
| Medium | Straina | The number of vacuoles/cellb | ||||
| 1 | 2 | 3 | 4 | 5 | ||
| % | % | % | % | % | ||
| SD | Wt | 68.6 (±5.76) | 27.1 (±5.15) | 1.74 (±1.43) | 0 (±0) | 0 (±0) |
| AA | 45.6 (±10.6) | 42.4 (±7.25) | 6.44 (±2.43) | 1.06 (±0.495) | 0.292 (±0.413) | |
| DD | 57.0 (±5.62) | 36.5 (±5.50) | 3.65 (±0.943) | 0.960 (±0.698) | 0.273 (±0.387) | |
| SD + 0.9 M NaCl | Wt | 27.9 (±9.90) | 42.7 (±4.63) | 20.6 (±5.25) | 5.60 (±2.72) | 2.32 (±1.33) |
| AA | 17.4 (±2.93) | 42.1 (±7.15) | 25.4 (±2.58) | 9.56 (±3.26) | 4.73 (±4.95) | |
| DD | 35.2 (±9.76) | 39.5 (±3.80) | 17.6 (±3.82) | 4.68 (±1.60) | 1.60 (±1.71) | |
The difference in the average of total vacuoles per cell between PpAtg18Wt and PpATG18AA was marginally significant (P < 0.1). Wt, wild type.
a
PpAtg18Wt, AA, or DD tagged 5×Flag were expressed under the PpATG18 promoter in Ppatg18Δ. Cells were grown in SD medium, and shifted to SD or SD + 0.9 M NaCl for 1 h.
b
Vacuolar fission indicated as the population of the cells (%) having the indicated number of vacuoles in a cell.