Table 3.
VSM formation during micropexophagy in PpAtg18 AA and DD mutant strains
| Straina | VSM formationb | ||||
| 1 | 2 | 3 | 4 | 5 | |
| % | % | % | % | % | |
| Wt | 55.5 (±5.14) | 30.1 (±3.95) | 10.7 (±1.54) | 2.94 (±1.03) | 0.178 (±0.182) |
| AA | 40.3 (±4.81) | 36.7 (±2.84) | 16.9 (±2.25) | 4.11 (±1.02) | 0.986 (±0.632) |
| DD | 45.1 (±6.45) | 36.6 (±1.26) | 13.6 (±4.27) | 3.09 (±1.76) | 0.260 (±0.261) |
The difference in the average of VSMs per cell between PpAtg18Wt and PpATG18AA was significant (P < 0.05). Wt, wild type.
a
PpAtg18Wt, AA, or DD tagged 5×Flag were expressed under the PpATG18 promoter in Ppatg18Δ. Micropexophagy was induced by adapting cells from methanol to glucose medium for 30 min
b
VSM formation is indicated as the population of the cells (%) having the indicated number of vacuoles in a cell.