Figure A1.

Characterization of the immature state of dendritic cells after 7 days of differentiation with GM-CSF and IL-4 as determined by flow cytometry. After 7 days of culture in the presence of GM-CSF and IL-4, DCs were labeled with the following antibodies: CD14-FITC, CD1a-FITC, CD40-PE, CD80-PE, CD83-FITC, CD86-PE, and HLA-DR-PECy5. They were analyzed by flow cytometry using a Canto II cytometer (Becton Dickinson, Le Pont de Claix, France). The percentages of positively labeled cells were determined after subtraction of the isotype control values (black histograms on the top of the figure) and are presented in gray histograms for each surface marker. In mature DCs, CD40, CD80, CD83, and CD86 were low in accordance with a non-activated stage. A high proportion of DCs expressed the CD1a and HLA-DR molecules. For each activation marker data [percentage of positive cells + mean fluorescence intensity (MFI)] are indicated. One representative experiment out of 13 is shown.