Figure A3.

Apoptosis rate in dendritic cell apoptosis activated by H. pylori. DCs were cocultivated for 48 h with B38 or B45 H. pylori strain at MOI 1. The cells were labeled with anti-annexin V-FITC antibody and propidium iodure and analyzed by flow cytometry. Percentages of viable DCs (double negative cells in Q3), necrotic DCs (propidium iodure positive-FITC negative in Q1), and apoptotic DCs (FITC-positive, propidium iodure ± in Q2 and Q4) are depicted in each area. The percentages are shown for one representative experiment out of 3 independent experiments (y-axis, propidium iodure; x-axis, anti-annexin V-FITC) (NI for uninfected DCs).