Table A1.
MiRNA microarray data in uninfected DC (control) and DCs infected for 48 h by H. pylori B38 strain at MOI 1.
| Mature miRNA ID | Ct control DC | Ct B38-infected | Rel value in control | Rel value in B38-infected | Fold change upon infection |
|---|---|---|---|---|---|
| hsa-let-7a | 21.6 | 18.34 | 2.7368 | 4.3169 | 1.5773 |
| hsa-let-7c | 29.79 | 26.4 | 0.0094 | 0.0162 | 1.7261 |
| hsa-let-7d | 25.72 | 22.11 | 0.1574 | 0.3164 | 2.0104 |
| hsa-let-7e | 24.03 | 19.17 | 0.5079 | 2.4284 | 4.7816 |
| hsa-let-7g | 21.28 | 17.74 | 3.4165 | 6.5432 | 1.9152 |
| hsa-miR-103 | 20.51 | 17.97 | 5.8260 | 5.5790 | 0.9576 |
| hsa-miR-106a | 19.89 | 18 | 8.9538 | 5.4642 | 0.6103 |
| hsa-miR-125a-5p | 19.32 | 17.27 | 13.2921 | 9.0631 | 0.6818 |
| hsa-mir-125b | 22.11 | 21.01 | 1.9219 | 0.6783 | 0.3529 |
| hsa-miR-126 | 22.34 | 21.36 | 1.6386 | 0.5322 | 0.3248 |
| hsa-miR-128 | 23.57 | 22.36 | 0.6986 | 0.2661 | 0.3809 |
| hsa-miR-130a | 30.73 | 27.14 | 0.0049 | 0.0097 | 1.9827 |
| hsa-miR-132 | 21.35 | 19.8 | 3.2546 | 1.5692 | 0.4821 |
| hsa-miR-135b | 33.42 | 28.11 | 0.0008 | 0.0049 | 6.5319 |
| hsa-miR-138 | 31.96 | 28.63 | 0.0021 | 0.0034 | 1.6558 |
| hsa-miR-142-3p | 18.17 | 15.83 | 29.4971 | 24.5900 | 0.8336 |
| hsa-miR-142-5p | 21.05 | 19.47 | 4.0069 | 1.9725 | 0.4923 |
| hsa-miR-143 | 28.96 | 27.05 | 0.0167 | 0.0103 | 0.6188 |
| hsa-miR-145 | 29.57 | 27.39 | 0.0109 | 0.0081 | 0.7461 |
| hsa-miR-146a | 20.87 | 16 | 4.5394 | 21.8566 | 4.8149 |
| hsa-miR-147 | 26.29 | 22.06 | 0.1060 | 0.3276 | 3.0898 |
| hsa-miR-148a | 23.17 | 21.27 | 0.9218 | 0.5664 | 0.6145 |
| hsa-miR-150 | 27.96 | 27.6 | 0.0333 | 0.0070 | 0.2113 |
| hsa-miR-152 | 24.84 | 24.51 | 0.2897 | 0.0600 | 0.2070 |
| hsa-miR-155 | 25.45 | 19.1 | 0.1898 | 2.5491 | 13.4310 |
| hsa-miR-15a | 22.35 | 19.39 | 1.6273 | 2.0849 | 1.2812 |
| hsa-miR-15b | 21.33 | 19.35 | 3.3001 | 2.1435 | 0.6495 |
| hsa-miR-16 | 15.39 | 14.69 | 202.6014 | 54.1917 | 0.2675 |
| hsa-miR-181a | 25.92 | 22.12 | 0.1370 | 0.3143 | 2.2934 |
| hsa-miR-182 | 29.4 | 27.82 | 0.0123 | 0.0060 | 0.4923 |
| hsa-miR-183 | 32.22 | 29.45 | 0.0017 | 0.0020 | 1.1231 |
| hsa-miR-184 | 34.48 | 29.77 | 0.0004 | 0.0016 | 4.3094 |
| hsa-miR-185 | 23.65 | 21.63 | 0.6609 | 0.4414 | 0.6678 |
| hsa-miR-186 | 24.85 | 21.41 | 0.2877 | 0.5141 | 1.7870 |
| hsa-miR-187 | 31.73 | 24.73 | 0.0024 | 0.0515 | 21.0756 |
| hsa-miR-18a | 21.9 | 20.46 | 2.2230 | 0.9931 | 0.4467 |
| hsa-miR-18b | 28.4 | 25.7 | 0.0246 | 0.0263 | 1.0699 |
| hsa-miR-191 | 18.39 | 17.18 | 25.3252 | 9.6465 | 0.3809 |
| hsa-miR-194 | 25.03 | 23.52 | 0.2539 | 0.1191 | 0.4689 |
| hsa-miR-195 | 15.87 | 15.22 | 145.2606 | 37.5307 | 0.2584 |
| hsa-miR-196a | 32.29 | 29.02 | 0.0017 | 0.0026 | 1.5883 |
| hsa-miR-198 | 34.73 | 29.2 | 0.0003 | 0.0023 | 7.6079 |
| hsa-miR-19a | 18.9 | 16.6 | 17.7839 | 14.4200 | 0.8108 |
| hsa-miR-19b | 19.13 | 16.88 | 15.1632 | 11.8762 | 0.7832 |
| hsa-miR-200a | 31.24 | 29.64 | 0.0034 | 0.0017 | 0.4991 |
| hsa-miR-203 | 28.95 | 27.92 | 0.0168 | 0.0056 | 0.3362 |
| hsa-miR-205 | 31.25 | 30.96 | 0.0034 | 0.0007 | 0.2013 |
| hsa-miR-206 | 32.49 | 28.21 | 0.0014 | 0.0046 | 3.1987 |
| hsa-miR-20a | 19.34 | 17.42 | 13.1091 | 8.1681 | 0.6231 |
| hsa-miR-20b | 20.8 | 19.06 | 4.7651 | 2.6208 | 0.5500 |
| hsa-miR-21 | 12.7 | 11.7 | 1307.4137 | 430.5390 | 0.3293 |
| hsa-miR-210 | 25.13 | 21.08 | 0.2369 | 0.6462 | 2.7274 |
| hsa-miR-214 | 31.61 | 26.95 | 0.0027 | 0.0110 | 4.1626 |
| hsa-miR-223 | 15.89 | 14.97 | 143.2608 | 44.6318 | 0.3115 |
| hsa-miR-23b | 22.38 | 20.89 | 1.5938 | 0.7371 | 0.4625 |
| hsa-miR-26a | 18.23 | 16.05 | 28.2955 | 21.1121 | 0.7461 |
| hsa-miR-26b | 18.8 | 16.95 | 19.0603 | 11.3137 | 0.5936 |
| hsa-miR-27a | 17.31 | 15.27 | 53.5383 | 36.2523 | 0.6771 |
| hsa-miR-27b | 22.41 | 20.95 | 1.5610 | 0.7071 | 0.4530 |
| hsa-miR-299-3p | 34.48 | 29.4 | 0.0004 | 0.0020 | 5.5693 |
| hsa-miR-29b | 28.19 | 23.49 | 0.0284 | 0.1216 | 4.2797 |
| hsa-miR-29c | 23.17 | 19.64 | 0.9218 | 1.7532 | 1.9020 |
| hsa-miR-302a | 34.03 | 29.83 | 0.0005 | 0.0015 | 3.0262 |
| hsa-miR-30b | 19.66 | 18.3 | 10.5013 | 4.4383 | 0.4226 |
| hsa-miR-30c | 19.39 | 18.5 | 12.6626 | 3.8637 | 0.3051 |
| hsa-miR-30e | 22.43 | 19.82 | 1.5395 | 1.5476 | 1.0052 |
| hsa-miR-335 | 28.9 | 27.56 | 0.0174 | 0.0072 | 0.4168 |
| hsa-miR-34a | 25.47 | 20.82 | 0.1872 | 0.7738 | 4.1339 |
| hsa-miR-363 | 25.96 | 24.12 | 0.1333 | 0.0786 | 0.5895 |
| hsa-miR-370 | 26.25 | 24.36 | 0.1090 | 0.0665 | 0.6103 |
| hsa-miR-409-3p | 26.52 | 26.45 | 0.0904 | 0.0156 | 0.1728 |
| hsa-miR-451 | 32.92 | 28.03 | 0.0011 | 0.0052 | 4.8821 |
| hsa-miR-493 | 31.14 | 29.19 | 0.0037 | 0.0023 | 0.6362 |
| hsa-miR-574-3p | 22.65 | 21.66 | 1.3218 | 0.4323 | 0.3270 |
| hsa-miR-9 | 25.47 | 22.79 | 0.1872 | 0.1975 | 1.0552 |
| hsa-miR-98 | 24.74 | 22.01 | 0.3105 | 0.3392 | 1.0924 |
| hsa-miR-99b | 21.24 | 19.79 | 3.5125 | 1.5801 | 0.4498 |
| SNORD48 | 26.92 | 23.15 | |||
| SNORD47 | 22.99 | 20.79 | |||
| SNORD44 | 19.91 | 18.54 | |||
| RNU6-2 | 22.39 | 19.32 | |||
| HKG mean Ct | 23.0525 | 20.45 |
One μg of total RNA from DCs cultured for 48 h in the absence or presence of H. pylori B38 at MOI 1 was retro-transcribed using the RT2 miRNA First Strand Kit (Qiagen, Courtaboeuf, France). The first strand reaction was mixed with RT2 SYBR Green qPCR mix and distributed onto PCR array plates containing primers for 88 miRNA involved in human immunopathology (SABioscience, Qiagen) according to the manufacturer’s protocol. The amplification profiles were measured on a Stratagene Mx3005P instrument (Life Technologies, Saint Aubin, France). The relative value for each miRNA was calculated by the Δ Ct method taking account the mean Ct for housekeeping genes (HKG). The fold induction of each miRNA corresponds to the ratio of its relative value in B38-infected DCs versus its relative value in control DCs.