Downregulated miRNA	Fold change upon infection	Relative initial level
hsa-miR-409-3p	0.1728	0.0904
hsa-miR-152	0.2070	0.2897
hsa-miR-150	0.2113	0.0333
hsa-miR-195	0.2584	145.2606
hsa-miR-16	0.2675	202.6014
hsa-miR-30c	0.3051	12.6626
hsa-miR-223	0.3115	143.2608
hsa-miR-126	0.3248	1.6386
hsa-miR-574-3p	0.3270	1.3218
hsa-miR-21	0.3293	1307.4137
hsa-miR-203	0.3362	0.0168
hsa-mir-125b	0.3529	1.9219

One μg of total RNA from DCs cultured for 48 h in the absence or presence of H. pylori B38 at MOI 1 was retro-transcribed using the RT2 miRNA First Strand Kit (Qiagen, Courtaboeuf, France). The first strand reaction was mixed with RT2 SYBR Green qPCR mix and distributed onto PCR array plates containing primers for 88 miRNA involved in human immunopathology (SABioscience, Qiagen) according to manufacturer's protocol. The amplification profiles were measured on a Stratagene Mx3005P instrument (Life Technologies, Saint Aubin, France). Data were analyzed by the C_t method (Livak and Schmittgen, 2001), using the average C_t of the housekeeping RNA for normalization. Data are expressed as fold induction compared to non-activated DCs in the medium lane. For each miRNA, the level relative to the mean expression of housekeeping genes is shown in the right lane, in order to assess which miRNA may be expressed at levels high enough to be relevant (highlighted).