Fig. 3.

Real-time PCR analysis of TLR expression in Müller cells. Total RNA was extracted from passage 4 Müller cells and cDNA was generated. The expression of TLRs was analyzed by real-time PCR using specific primers. The concentration of the genes of interest was determined using the comparative threshold cycle number and normalized to that of the GAPDH (amount of target = 2^−∆CT %). Representative results from three independent experiments are shown