Table 2.
F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 | |
---|---|---|---|---|---|---|---|---|---|
Coding of variables | |||||||||
X 1 level | −1 | 0 | +1 | −1 | 0 | +1 | −1 | 0 | +1 |
X 2 level | −1 | −1 | −1 | 0 | 0 | 0 | +1 | +1 | +1 |
Ingredients | |||||||||
AA* (50% v/v) (μL) | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 |
Chitosan (1% w/v) (μL) | 100 | 200 | 300 | 100 | 200 | 300 | 100 | 200 | 300 |
HPMC† K4M (2% w/v) (μL) | 100 | 100 | 100 | 200 | 200 | 200 | 300 | 300 | 300 |
BIS‡ (2.5% w/v) (μL) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
TEMED§ (20% v/v) (μL) | 25 | 25 | 25 | 25 | 25 | 25 | 25 | 25 | 25 |
APS|| (20% w/v) (μL) | 25 | 25 | 25 | 25 | 25 | 25 | 25 | 25 | 25 |
Span 80 (10% v/v) (μL) | 30 | 30 | 30 | 30 | 30 | 30 | 30 | 30 | 30 |
DDW¶ (μL) | 300 | 300 | 300 | 300 | 300 | 300 | 300 | 300 | 300 |
Sodium bicarbonate (mg) | 200 | 200 | 200 | 200 | 200 | 200 | 200 | 200 | 200 |
*AA: acrylic acid; †HPMC: hydroxypropylmethyl cellulose; ‡BIS: N,N′-methylene-bis-acrylamide; §TEMED: N,N,N′,N′-tetramethylethylenediamine; ||APS: ammonium persulfate; ¶DDW: double distilled water.