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. 2001 Jul 3;98(15):8554–8559. doi: 10.1073/pnas.141230798

Figure 2.

Figure 2

MetAP-2 binds Protac specifically and in a concentration-dependent manner. (A) MetAP-2 (9 μM) was incubated with increasing concentrations of Protac-1 at room temperature for 45 min. The last two lanes depict MetAP-2 that was incubated with either free IPP (50 μM) or free OVA (50 μM), as indicated. After incubation, samples were supplemented with SDS/PAGE loading buffer, separated by SDS/PAGE, and immunoblotted with MetAP-2 antiserum. (B) Same as A, except MetAP-2 (9 μM) plus Protac-1 (10 μM) were supplemented with either IPP (50 μM) or OVA (50 μM), as indicated. Protac binding to MetAP-2 was inhibited by the addition of OVA, but not by the addition of IPP.