Figure 2.

Histological observation of adventitious shoot formation from leaf explant of L. serratus, bar = 500 μm. (a) Leaf explant before culture initiation. (b) Cell divisions in the adaxial leaf epidermis and close to the vascular bundles (arrowheads) 10 days after culture initiation on medium containing 0.5 mg L⁻¹ BA. (c) Cell division to form adventitious shoot meristems (M) from explant (Ex) 20 days after culture initiation on medium containing 0.5 mg L⁻¹ BA. (d) Adventitious shoot with leaf primordial (L) and shoot meristems (M) formed, with vascular bundles (arrowheads) connected to explant (Ex) 30 days after culture on medium containing 0.5 mg L⁻¹ BA and 0.05 mg L⁻¹ NAA. Ca indicates callus at the cutting edge.