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Leucylation carried out by 5 nM SpLeuRS or its mutants including T252R, Y332D (a), and SpLeuRS-MmLinker (b). Symbols are 
, 
, 
, and 
, respectively, in the absence of ZCL039; 
, 
, 
and 
, respectively, in the presence of 50 μM ZCL039. Data are means from three independent experiments; errors bars show s.d.
