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. 2013 Aug 20;3:2474. doi: 10.1038/srep02474

Figure 7. 14-3-3zeta-mediated RPN2 upregulation is required for the mtp53 stability.

Figure 7

(a) Detected peptides that differed between RPN2 knockdown cell line (MM231-LN shRPN2) and control (MM231-LN shNC). MS peak of 14-3-3Zeta (1433Z) in MM231-LN shNC (Right) and MM231-LN shRPN2 (Left) (indicated by arrows). (b) Gel-like views of MS peaks with retention time (RT) along the vertical axes (top) and distribution of the mean peak intensity of duplicates (bottom) across 6 samples. (c) Spot intensity of 14-3-3Z in MM231-LN shNC and MM231-LN shRPN2. (d) 14-3-3zeta reduced wtp53 expression. Lysates from MCF7 cells, transfected as indicated, were immunoblotted as shown. (e) and (f) RPN2 stabilized mtp53 in MM231-LN cells. Lysates from MM231-LN cells, transfected as indicated, were immunoblotted as shown. (g) and (h) 14-3-3zeta-dependent feedback regulation of mtp53 protein stability. Full-length gels and blots are shown in supplementary figure 17 and 18.