. Author manuscript; available in PMC: 2013 Aug 20.

Published in final edited form as: J Endocrinol. 2009 Jul 8;203(1):75–85. doi: 10.1677/JOE-09-0179

Table I.

OBRa, OBRb, and OBRc steady-state mRNA expression^a,^b

	OBRa	OBRb	OBRc
Whole Thymus	10.52 ± 0.71	18.08 ± 1.04	11.92 ± 2.59
Enriched Thymus Stromal Fraction	5.15 ± 1.03	12.92 ± 0.73	8.60 ± 0.71
Thymocyte Fraction	15.78 ± 2.59	> 20.0	11.05 ± 0.95
CD4+ SP Thymocytes	17.53 ± 2.43	> 20.0	10.53 ± 0.31
CD4+CD8+ (DP) Thymocytes	18.59 ± 1.41	> 20.0	9.67 ± 0.72
CD8+ SP Thymocytes	18.87 ± 1.38	> 20.0	10.28 ± 1.23
CD4-CD8- (DN) Thymocytes	16.83 ± 1.89	> 20.0	11.70 ± 1.85

Cycle Threshold (C_t) values normalized to GAPDH (mean ± SD, n = 3)

Normalized C_t values > 20.0 correlate to OBR C_t values > 40.0 cycles, and are considered to be undetectable.