TABLE 1 .
Oligonucleotides used in this study
| Primer | Sequence (5′–3′)a | Used in this work |
|---|---|---|
| CspA BamHI | GCTAAAACTTCTCTTTTTTTTAGGATCCCAACCCAAATCC | Cloning in pKFSS1 |
| CspA HindIII | GAAAGAAAAAAAATAAGCTTTTGCACTTGATATTTTTAAAAAG | Cloning in pKFSS1 |
| pGEX-CspA75 | CGGATCTGGTTCCGCGTGGATCCGCACCTTTTAGC | Recloning in pQE-30 Xa |
| BbCspA 175(+) | ACCCAAAATTTTGAAGATAAATCTGGATCCCTTAGCACTTCTGATGAA | Recloning in pQE-30 Xa |
| pGEX(−) | CCGGGAGCTGCATGTGTCAGAGG | Recloning in pQE-30 Xa |
a
Nucleotides underlined indicate the appropriate restriction sites for cloning of the PCR-amplified cspA gene of B. burgdorferi LW2 into the shuttle vector pKFSS1 or recloning of truncated CspA genes encoding diverse CspA deletion mutants into the pQE-30 Xa expression vector (see Materials and Methods).