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. 2013 Aug 13;4(4):e00540-13. doi: 10.1128/mBio.00540-13

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Copyright © 2013 Ricci et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 3 — The surA10 and surA13 suppressors restore detergent resistance but differentially influence OMP levels. (A) Whole-cell extracts of strains indicated were prepared by boiling after harvesting cells at an OD₆₀₀ of 1. Major OMPs were detected by SDS-PAGE and immunoblotting. Detergent sensitivity of each strain is shown below the corresponding lane (SDS^R, detergent resistant; SDS^S, detergent sensitive). (B) β-Galactosidase activity was measured in the indicated strains containing a transcriptional fusion with the σ^E-dependent rpoH_P3 promoter driving expression of lacZ. (C) The strains indicated were processed as described for panel A, and the indicated envelope proteins were detected by immunoblotting.