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. 2013 Aug 13;4(4):e00540-13. doi: 10.1128/mBio.00540-13

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Copyright © 2013 Ricci et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 4 — The surA10 mutation does not restore the SurA-BamA⁶¹⁶ interaction. DSP cross-linking and affinity purification were performed using His₆-tagged BamA⁺ (lanes 1, 4, and 5) or BamA⁶¹⁶ (lanes 2 and 3). All strains indicated were incubated with the cross-linker DSP prior to lysis. Whole-cell extracts (left) and Ni-NTA-enriched eluate fractions (right) were subjected to SDS-PAGE and immunoblotting for BamA and SurA.