FIG 6 .

SurA^S220A is hyperactive in vivo and unstable in vitro. (A) Western blot analysis was performed with wild-type and surA10 strains to determine relative levels of SurA, LamB, and OmpA. (B) Serial 10-fold dilutions of stationary-phase cultures of ∆surA strains containing plasmids expressing SurA⁺, SurA^S220A, SurA^∆P1, or SurA^∆P2 were spotted onto LB with or without bacitracin and incubated at 37°C. (C) Periplasmic contents in cultures of wild-type and surA10 strains were released by chloroform shock, and samples were incubated at 37°C prior to SDS-PAGE. SurA and the periplasmic protein MalE were detected by Western blotting using the appropriate antisera.