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Scores scatter plot of (a) PC1 versus PC2 and (b) component 1 versus component 2 resulting from PCA and PLS-DA, respectively, applied to 1H NMR spectra of serum of endometriosis patients (black diamond) and controls (white diamond). Validation of the PLS-DA model was performed by (c) receiver operating characteristic (ROC) analysis where area under the curve (AUC) was found to be 0.99 (d) Permutation test statistics at 1000 permutations with observed statistic at P < 0.001.
