Table 1.
Differentially expressed membrane proteins, as identified by LC-MS/MS between of fluconazole-susceptible and -resistant C glabrata strains
| Spot | Protein | Molecular mass (kDa) | pI | Protein coverage (%) | Fold change (R/S)* |
|---|---|---|---|---|---|
| 151 | Ugp1 | 55.916 | 6.98 | 18 | –4.04 |
| 86 | Phosphoglycerate kinase | 44.590 | 6.37 | 8 | –2.33 |
| 65 | Malate dehydrogenase | 35.544 | 8.93 | 4 | –2.02 |
| 149 | Putative stress response protein | 59.391 | 5.93 | 15 | 3.98 |
| 26 | Nucleotide transport and metabolism hydrolase | 25.680 | 5.96 | 15 | 3.07 |
| 153 | Aldehyde dehydrogenase | 27.079 | 5.77 | 5 | 3.89 |
| 176 | Heat shock protein 70 | 69.806 | 5.06 | 13 | 2.98 |
| 72 | Glyceraldehyde 3-phosphate dehydrogenase | 35.871 | 6.46 | 2 | 2.12 |
| 136, 47 | Methionine aminopeptidase | 44.307 | 6.48 | 4 | 1.92 |
*Expression ratio of fluconazole-resistant (R) over fluconazole-susceptible (S) strains. The minus sign (-) indicatesthe decreased protein expression of fluconazole-resistant strains in comparison with fluconazole-susceptible strains.
LC-MS/MS: liquid chromatography-tandem mass spectrometry.