Table 2.
Differentially expressed cellular proteins, as identified by LC-MS/MS, between fluconazole-susceptible and -resistant C glabrata strains
| Spot | Protein | Molecular mass (kDa) | pI | Protein coverage (%) | Fold change (R/S)* |
|---|---|---|---|---|---|
| 288 | Unnamed protein | 61.726 | 5.59 | 10 | 1.90 |
| 64 | Fructose bisphosphate aldolase | 39.322 | 5.49 | 4 | 1.90 |
| 82 | Phosphoglycerate kinase | 44.590 | 6.37 | 16 | 1.71 |
| 286 | GroEL-like type I chaperonin | 60.351 | 5.14 | 5 | –2.50 |
| 287, 290, 217, 284, 248 | Heat shock protein70 | 66.355 | 5.32 | 5 | –2.31 |
| 272, 270, 273 | Hexokinase | 53.772 | 5.23 | 32 | –2.30 |
| 187 | Putative oxidoreductase | 33.032 | 5.01 | 4 | –2.15 |
| 282, 250 | Putative pyruvate decarboxylase | 61.531 | 5.43 | 6 | –2.09 |
| 281 | Pyruvate kinase | 55.060 | 6.08 | 6 | –2.03 |
| 41 | DNA-dependent RNA polymerase II | 38.917 | 7.7 | 7 | –1.95 |
| 141 | Mitochondrial aconitase hydratase | 84.169 | 5.96 | 1 | –1.83 |
| 138 | CoA-transferase | 57.167 | 8.46 | 5 | –1.75 |
| 176 | IPP1P inorganic pyrophosphatase | 14.532 | 4.84 | 83 | –1.66 |
*Expression ratio of fluconazole-resistant (R) over fluconazole-susceptible (S) strains. The minus sign (-) indicates decreased protein expression of fluconazole-resistant strains in comparison with fluconazole-susceptible strains.
LC-MS/MS: liquid chromatography-tandem mass spectrometry.