Table 1.
Characteristics of EAEC isolates with resistance to fluoroquinolones
| MIC (μg/mL) | gyrA amino acid at codon | parC amino acid at codon | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 83 | 87 | 157 | 80 | 81 | 84 | ||||||
| Strain no. | CIP | CIP + Reserpine | Nor | Nor + Reserpine | GAC (Asp) | GAC (Asp) | CGT (Arg) | AGC (Ser) | GCC (Ala) | GAA (Glu) | qnr, aac(6’)Ib-cr, qepA type |
| EACR01 | 64 | <2 | 32 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | -T-(Val) | |
| EACR02 | 64 | <2 | 32 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | -T-(Val) | |
| EACR03 | 64 | <2 | 32 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | -T-(Val) | |
| EACR04 | 64 | <2 | 32 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | -T-(Val) | |
| EACR05 | 64 | <2 | 32 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | -T-(Val) | |
| EACR06 | 64 | <2 | 32 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | -T-(Val) | |
| EACR07 | 64 | <2 | 32 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | – | |
| EACR09 | 64 | <2 | 32 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | -T-(Val) | |
| EACR10 | 64 | <2 | 32 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | -T-(Val) | |
| EACR11 | 64 | <2 | 32 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | -T-(Val) | |
| EACR12 | 64 | <2 | 32 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | -T-(Val) | |
| EACR15 | 32 | <2 | 32 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | – | |
| EACR17 | 32 | <2 | 32 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | -T-(Val) | |
| EACR18 | 64 | <2 | 32 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | -T-(Val) | |
| EACR21 | 64 | <2 | 32 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | -T-(Val) | |
| EACR22 | 64 | <2 | 32 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | -T-(Val) | |
| EACR23 | 64 | <2 | 32 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | -T-(Val) | |
| EACR24 | 32 | <2 | 32 | <2 | -T-(Leu) | – | – | – | C–(Pro) | – | |
| EACR25 | 128 | <2 | 32 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | – | aac(6’) Ib-cr |
| EACR29 | 256 | <2 | 64 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | – | aac(6’) Ib-cr |
| EACR31 | 256 | <2 | 64 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | – | |
| EACR32 | 128 | <2 | 64 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | – | aac(6’) Ib-cr |
| EACR33 | <256 | <2 | 256 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | -G-(Gly) | qnrA |
| EACR38 | 128 | <2 | 32 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | – | |
| EACR39 | 128 | <2 | 64 | <2 | -T-(Leu) | -G-(Gly) | G-(Gly) | -AG(Gln) | – | – | |
| EACR41 | 64 | <2 | 32 | <2 | -T-(Leu) | -G-(Gly) | – | -AG(Gln) | – | -G-(Gly) | |
Each value is the average of five replicates.
CIP = ciprofloxacin; NOR =norfloxacin.