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. Author manuscript; available in PMC: 2013 Aug 20.
Published in final edited form as: J Pharm Sci. 2008 Sep;97(9):3518–3590. doi: 10.1002/jps.21270

Figure 6.

Figure 6

Kinetic considerations at nanovehicle uptake and exocytosis in vitro. The overall fate could be traced by flow cytometry (FACS) following the cell detachment via trypsin or EDTA (ethylenediamine tetraacetic acid). Nanovehicles must be present in medium in excess to be internalized. The efflux could be inhibited by specific inhibitors. The quantity of nanovehicles could be distinguished from total uptake by fluorescence quenching by Trypan Blue (A). When resuspended in fresh media without nanovehicles, they can efflux to the environment. This process requires energy and presence of serum (B).