Table 5.

Approved and most frequently used rapid tests for the detection of BSE [19,63,64]

Product name (company)	Diagnostic method	Authorized country	Note
Prionics-Check	Western blot using the proteinase–processed specimen using an antigen to prion	EU	Specimen: 0.5 g obex tissue
Western test (Prionics AG)		USA	Limit: ∼5.0–20 pmol
			Test period: ∼6–8 h
Prionics-Check LIA (Prionics AG)	Sandwich ELISA	EU	Specimen: 0.5 g obex tissue
		USA	Limit: ∼1.0–5.0 pmol
			Test period: 4 h
Bio-Rad test (Bio-Rad)	Sandwich ELISA	EU	Specimen: 0.35 g obex tissue
		USA	Limit: ∼0.5–2.0 pmol
		Japan	Test period: 6 h
Enfer test (Abbott Labs)	Simple ELISA	EU	Limit: ∼1–10 pmol
		USA	Test period: 4 h
		Japan
CDI test (Impro Biotechnology)	Immunity analysis based on the structural differences	EU	Limit: ∼0.5–5.0 pmol
		USA	Test period: 8 h
		Japan	No proteinase K
Herd-check (IDEXX Laboratories)	Immunity analysis after separating the variant prion (PrPSc) from brain tissue specimens	USA	Test period: ∼4–5hours
			No proteinase K

ELISA = enzyme-linked immunosorbent assay.