Table 1.

Dissociation constants of anti-guanine-quadruplex scFvs determined by inhibition BIAcore and definition of the minimal DNA epitope bound

DNA sequence	Kd, nM
	scFv Sty3	scFv Sty49
4⋅d(TG4T) parallel	654  ± 56	249  ± 55
4⋅d(T2G4T) parallel	117  ± 17	104  ± 32
4⋅d(T3G4T) parallel	1.38  ± 0.16	26.1  ± 5.3
4⋅d(T4G4T) parallel	0.126  ± 0.011	2.96  ± 0.53
4⋅d(T4G3T) parallel	7.0  ± 0.9	61.7  ± 7.7
d(T4G2T)*	—†	—†
d(G4T4G4T4G4T4G4) antiparallel	171  ± 14	5.08  ± 1.69

^*d(T₄G₂T) does not adopt a quadruplex structure under the conditions studied (33). 

^† No binding detected.