Figure 1. .

Immunofluorescence of TNC in trabecular meshwork tissue. Human anterior segments were cultured at physiological pressure (D–H) or at 2× pressure (A–C) for 24 hours. Tissue was fixed; frontal sections were cut and immunostained with TNC (red) and fibrillin-1 antibodies (green) (A–H). Single channels are shown for each protein, and a merged image with DAPI-stained nuclei is also shown (C, F, H). Higher-magnification images of boxed regions are also shown (G, H). Note that the bright spherical dots in the red channel may be Qdots (white stars), since these images were from tissue labeled for low segmental outflow. Size of JCT parentheses marker is 14 μm. OW, outer wall; IW, inner wall; SC, Schlemm's canal; JCT, juxtacanalicular region. Scale bars: 10 μm.