Figure 5. .
TNC mRNA and protein levels in response to elevated pressure. (A) Human eyes were perfused at physiological or 2× elevated pressure for 24 hours. RNA was isolated from TM tissue, and the TNC mRNA levels were quantitated using qRT-PCR and normalized for GAPDH mRNA levels. Error bars signify the standard error of the mean. *P = 0.047 by a Student's t-test, n = 5. (B) Western immunoblots of tenascin C and fibronectin in RIPA extracts of TM tissue dissected from anterior segments perfused at physiological or 2× pressure for 48 hours. The immunoblots were simultaneously probed with both antibodies. Three replicates are shown for each treatment. (C) Quantitation of tenascin C and fibronectin bands by densitometry. Error bars signify the standard error of the mean. *P = 0.047; n = 3.