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SUV-SBL fusion. (A) Far-field epifluorescence. Frames are 100 ms apart. (B) TIRFM. Frames are 17 ms apart. Note that individual LR-PE lipid labels become discernible as they diffuse sufficiently apart from one another. Their mobility can be quantified from single-molecule tracking, which yields a diffusivity of D ≈ 2 μm2/s9.
