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. Author manuscript; available in PMC: 2013 Aug 20.
Published in final edited form as: Nat Protoc. 2012 Apr 19;7(5):903–920. doi: 10.1038/nprot.2012.019

Figure 7.

Figure 7

Analysis of data using PointPicker and MatLab. The data shown here were acquired using far-field epifluorescence with a time resolution of 100 ms. (A) Screenshot of PointPicker. Clicking the mouse on a vesicle marks it with a cross whose x, y and frame coordinates are saved into a text file. PointPicker files from many movies are read and analyzed in batch using the MatLab programs EK_SUVSBL_Fdotbatch.m and EK_SUVSBL_Ddotbatch.m which calculate the survival probability beyond a given delay after docking (B), the cumulative docking and fusion events as a function of time (C) and the averaged docking and fusion rates (D). See text and the supplementary material for more information.